Generator

Part:BBa_K5319688:Design

Designed by: Zhang Liting   Group: iGEM24_ZJU-China   (2024-10-01)


VioE expression circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 301
    Illegal AgeI site found at 1354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 531
    Illegal BsaI site found at 975


Design Notes

There will be leakage expression in Psal.


Source

VioE is derived from ATCC 21850.

References